LC-MS ANALYSIS OF VITIS SP ROOTSTOCKS CO-CULTIVATED WITH FUSARIUM OXYSPORUM AT DIFFERENT TIMES.

Igor Rafael Sousa Costa1,2,3, Elenilson de Godoy Alves Filho1,4, Kirley Marques Canuto5, Paulo Riceli Vasconcelos Ribeiro5, Fábio Rossi Cavalcanti6, Cléberson de Freitas Fernandes5, Humberto Henrique de Carvalho1,2,3

1. UFC, Universidade Federal do Ceará; Av. Mister Hull, s/n - Pici - CEP 60455-760 - Fortaleza - CE
2. DBBM, Department of Biochemistry and Molecular Biology; Rua Campus do Pici, Centro de Ciências, s/n - Bloco 907, Fortaleza - CE, 60440-900
3. INCTAgriS , Instituto Nacional de Ciência e Tecnologia em Agricultura Sustentável no Semiárido Tropical; Fortaleza, Ceará, Brazil
4. DEAL, Department of Food Engineering; Rua Campus do Pici, Bloco 858, CEP 60440-900, Fortaleza, CE, Brazil.
5. Embrapa Agroindustria Tropical, Empresa Brasileira de Pesquisa Agropecuária; Rua Dra Sara Mesquita, 2270, Pici, 60511-110 Fortaleza, CE, Brazil.
6. Embrapa Uva e Vinho, Empresa Brasileira de Pesquisa Agropecuária; Rua Livramento 515, Juventude, 95700000 Bento Gonçalves, RS – Brasil

Grapevines are one of the main fruit crops in the world and are susceptible to many infections, especially fungi such as Fusarium oxysporum. The search for genotypes and the use of more tolerant rootstocks have become a strategy to minimize this problem. Thus, the objective of this work was to evaluate the secondary metabolic profiles of rootstocks (Paulsen 1103 and BDMG573) co-cultivated with F. oxysporum using a non-targeted global metabolomics approach and thus identify different compounds  at different times of exposure. Grapevine leaf extracts were evaluated by Liquid Chromatography coupled to Mass Spectrometry (LC-MS) in a positive ionization mode. Samples were collected from susceptible and tolerant grapevines genotypes at three different times: 0, 4 and 8 days after treatment. A total of 23 compounds were identified in leaves of grapevine susceptible (BDMG573 genotype) and tolerant (P1103 ). The Partial Least Squares Discriminant Analysis (PLS-DA)of P1103 demonstrated a cluster with positive LV1 scores, due to the amounts of caftaric acid, catechin , coutaric acid, vescalin/castalin, feruloyltartaric acid, p-coumaroyltartaric acid, tri-O-galloyl-O-glucose and quercetin-O-glucuronide. On the other hand, BDMG573 presented negative LV1 scores due to the high amounts of tartaric acid, resveratrol dimer, alfa-viniferin, trihydroxyoctadecaenoic acid, anthracenecarboxylic acid and an unknown compound showing a clear separation of the groups. Overall, the modulation of secondary metabolites in tolerant and sensitive genotypes of grapevines to the fungus F. oxysporum was evident in relation to those of infection. Thus, it is expected that these data can contribute to assisted selection strategies to increase tolerance in different phases of infection, as well as early identification and mechanisms of the disease response.

Agradecimentos: Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará, Brazil. Embrapa Agroindustria Tropical, Fortaleza, CE, Brazil. Embrapa Uva e Vinho, Bento Gonçalves, RS, Brazil. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)